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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Robust and highly efficient hiPSC generation from patient non-mobilized peripheral blood-derived CD34+ cells using the auto-erasable Sendai virus vector

Fig. 3

Assessment of SeVdp(KOSM)-302L removal and karyotypes. a Gel images showing representative assessment results for the removal of the SeVdp(KOSM)-302L genomes obtained by RT-PCR. The image demonstrates no detection of SeVdp(KOSM)-302L amplicons in all 9 TkPP2 lines, whereas it shows positive results in 4 out of 6 TkSCG4 lines (at passages 5–6). Residual SeVdp(KOSM)-302L was no longer detected in these 6 TkSCG4 lines when assessed a few passages later (additional culture). SeVdp(−), negative control (uninfected hiPSC); SeV(+), low-positive control (hiPSC carrying low copies); SeV(++), high-positive control (hiPSC carrying high copies). GAPDH was used as the internal control. b Representative G-banded karyotyping images for 3 TkPP2 lines and 3 TkSCG4 lines. c Summary of the assessments of SeVdp(KOSM)-302L removal and G-band karyotypes. hiPSC, human induced pluripotent stem cells; N.D., not done

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Stem Cell Research & Therapy

ISSN: 1757-6512