Table 3 Colony formation efficiencies and number of established lines
Healthy donor and patients (hiPSC line) | Colony formation efficiencies (No. of colonies/No. of seeded cells) | No. of colonies per 1 mL blood | No. of lines established | Recovery success rate from frozen stock (No. of lines checked) |
|---|---|---|---|---|
Healthy (TkPP2) | 5.58% (781/14,000) | – | 10 | 100% (2) |
SLE (TkSLE3) | 2.61% (339/13,000) | 38.5 | 12 | 100% (6) |
SLE (TkSLE4) | 0.08% (12/16,000) | 0.65 | 4 | 100% (4) |
SLE (TkSLE5) | 3.26% (1283/39,375) | 171.1 | 12 | 100% (6) |
PM (TkSPD3) | 1.67% (130/7750) | 17.4 | 12 | 100% (5) |
PM (TkSPD4) | 3.17% (246/7750) | 59.1 | 14 | 100% (3) |
PM (TkSPD5) | 0.10% (16/14,000) | 1.8 | 12 | 100% (6) |
X-CGD (TkSCG3) | 0.29% (23/7750) | 1.7 | 12 | 100% (6) |
X-CGD (TkSCG4) | 0.26% (23/9000) | 2.7 | 12 | 100% (6) |
PID (TkSPR1) | 2.43% (438/18,000) | 83.2 | 12 | 100% (5) |
PID (TkSPR2) | 2.50% (294/11,750) | 38.1 | 12 | 100% (6) |
JIA (TkSCR1) | 1.59% (530/33,375) | 126.7 | 11 | 100% (6) |
CMS (TkS42–1) | 0.34% (17/5000) | 1.2 | 14 | 100% (6) |
MD (TkSmD1) | 1.09% (85/7800) | 4.6 | 26 | 100% (7) |
MD (TkSmD3) | 1.09% (215/19,763) | 45.9 | 36 | 100% (13) |
KCS2 (TkSKCII2) | 0.15% (43/27,750) | 8.9 | 12 | 100% (2) |