Table 1 Culture formula optimization for human CD34+ cell expansion
From: Safety and efficacy of ex vivo expanded CD34+ stem cells in murine and primate models
Group | IMDM | Nutrition Supplements | SCF (ng/mL) | Flt-3 L (ng/mL) | TPO (ng/mL) | IL-3 (ng/mL) | G-CSF (ng/mL) | GM-CSF (ng/mL) | SR1 (μM) |
|---|---|---|---|---|---|---|---|---|---|
GF0 | + | – | – | – | – | – | – | – | – |
SGF0 | + | + | – | – | – | – | – | – | – |
SGF2 | + | + | 200 | 200 | 0 | – | – | – | – |
SGF3 | + | + | 200 | 200 | 5 | – | – | – | – |
+ | + | 10 | – | – | – | – | |||
+ | + | 20 | – | – | – | – | |||
+ | + | 50 | – | – | – | – | |||
+ | + | 100 | – | – | – | – | |||
SGF4 | + | + | 200 | 200 | 20 | 5 | – | – | – |
+ | + | 10 | – | – | – | ||||
+ | + | 15 | – | – | – | ||||
+ | + | 25 | – | – | – | ||||
+ | + | 50 | – | – | – | ||||
+ | + | 100 | – | – | – | ||||
SGF5 | + | + | 200 | 200 | 20 | 15 | 5 | – | – |
+ | + | 10 | – | – | |||||
+ | + | 15 | – | – | |||||
+ | + | 25 | – | – | |||||
+ | + | 50 | – | – | |||||
+ | + | 100 | – | – | |||||
SGF6 | + | + | 200 | 200 | 20 | 15 | 10 | 5 | – |
+ | + | 10 | – | ||||||
+ | + | 15 | – | ||||||
+ | + | 25 | – | ||||||
+ | + | 50 | – | ||||||
+ | + | 100 | – | ||||||
SGF7 | + | + | 200 | 200 | 20 | 15 | 10 | 10 | 0.5 |
+ | + | 1 | |||||||
+ | + | 2 | |||||||
HEM | + | + | 200 | 200 | 20 | 15 | 10 | 10 | – |