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Table 1 Culture formula optimization for human CD34+ cell expansion

From: Safety and efficacy of ex vivo expanded CD34+ stem cells in murine and primate models

Group IMDM Nutrition Supplements SCF (ng/mL) Flt-3 L (ng/mL) TPO (ng/mL) IL-3 (ng/mL) G-CSF (ng/mL) GM-CSF (ng/mL) SR1 (μM)
GF0 +
SGF0 + +
SGF2 + + 200 200 0
SGF3 + + 200 200 5
+ + 10
+ + 20
+ + 50
+ + 100
SGF4 + + 200 200 20 5
+ + 10
+ + 15
+ + 25
+ + 50
+ + 100
SGF5 + + 200 200 20 15 5
+ + 10
+ + 15
+ + 25
+ + 50
+ + 100
SGF6 + + 200 200 20 15 10 5
+ + 10
+ + 15
+ + 25
+ + 50
+ + 100
SGF7 + + 200 200 20 15 10 10 0.5
+ + 1
+ + 2
HEM + + 200 200 20 15 10 10
  1. The composition of the base (GF0 and SGF0), intermediate (SGF2-SGF7), and the final optimized media formula (HEM) are shown