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Table 2 Primer sequences used for RT-PCR

From: Human predecidual stromal cells are mesenchymal stromal/stem cells and have a therapeutic effect in an immune-based mouse model of recurrent spontaneous abortion

mRNA Oligonucleotide primers PCR product size (bp)
ATP-binding cassette subfamily G member 2 (ABCG2) 5′-CCCATCCTGACCTCCAGCCG-3′ (F)
5′-TTGAGTGGGCACAGCACGCA-3′ (R)
127
Alkaline phosphatase (AP) 5′-TGGAGCTTCAGAAGCTCAACACCA-3′ (F)
5′-ATCTCGTTGTCTGAGTACCAGTCC-3′ (R)
338
β2-microglobulin 5′-CTCGCGCTACTCTCTCTCTTTCTGG-3′ (F)
5′-TCTACATGTCTCGATCCCACTTAA-3′ (R)
335
Collagen II 5′-TTTCCCAGGTCAAGATGGTC-3′ (F)
5′-CTTCAGCACCTGTCTCACCA-3′ (R)
159
Human cytochrome B (Cyt B) 5′-CCCATACATTGGGACAGACC-3′ (F)
5′-GACGGATCGGAGAATTGTGT-3′ (R)
394
Murine Cyt B 5′-TCGCGGCCCTAGCAATCGTT-3′ (F)
5′-TGGTTGGCCCCCAATTCAGGT-3′(R)
461
NANOG 5′-TGCTGGACTGAGCTGGTTGCC-3′ (F)
5′-AGCAAGGCAAGCTTTGGGGACA-3′ (R)
221
Octamer-binding transcription factor 4 (OCT-4) Oct4F: 5′-AGCCCTCATTTCACCAGGCC-3′(F)
Oct4FP: 5′-GATGGCGTACTGTGGGCCC-3′ (F)
Oct4R: 5′-TGGGACTCCTCCGGGTTTTG-3′ (R)
456
195
Osteopontin 5′-CTAGGCATCACCTGTGCCATACC-3′ (F)
5′-CAGTGACCAGTTCATCAGATTCATC-3′ (R)
373
Peroxisome proliferator-activated receptor γ2 (PPARγ2) 5′-GCTGTTATGGGTGAAACTCTG-3′ (F)
5′-ATAAGGTGGAGATGCAGGCTC-3′ (R)
351
  1. F forward, R reverse