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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Labeling of human mesenchymal stem cells with different classes of vital stains: robustness and toxicity

Fig. 2

Microscopic analysis of labeled hBM-MSC. a Vital observation of hBM-MSC stained with Molday ION Rhodamine B™ (Molday), CellTracker™ Green CMFDA (CMFDA), and eGFP (mRNA GFP) over 7 days in vitro culture. *DIV, day in vitro. Scale 50 μm. Measurement of fluorescence signal intensity generated by cells stained with b Molday ION Rhodamine B™ (Molday), c CellTracker™ Green CMFDA (CMFDA), and d transfected with mRNA eGFP on the second and seventh day of in vitro culture. e Comparison of percentage of fluorescent hBM-MSC in all groups. The viability of cells assessed in 7AAD test on the second and seventh day after labeling (f). The measurement of relative size (g) and granularity (H) of cells. *p < 0.05, **p < 0.01, ***p < 0.001 (n = 3–4)

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Stem Cell Research & Therapy

ISSN: 1757-6512