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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Hedgehog pathway inhibition causes primary follicle atresia and decreases female germline stem cell proliferation capacity or stemness

Fig. 5

Attenuating Hh signaling decreased FGSC proliferation in vitro. a CCK-8 assay was used to measure the proliferation activity of FGSCs cells after GANT61 treatment. b Change in ALP activity of FGSCs after different GANT61 dosages. c FGSC colony formation assays. d, e EdU staining combined with IF of Mvh and Oct4 comparing the fluorescence intensity between the control and GANT61 10 μM groups, scale is 20 μm. f FGSC proliferation activity was evaluated by EdU-positive cell rate. g mRNA expression levels of Gli1, Mvh, and Oct4 after GANT61 treatment. h, i Protein expression levels of Gli1, Mvh, and Oct4. j, k Dual-IF was used to detect the coexpression of Oct4 and Mvh in the control and GANT61 10 μM groups, the scale is 20 μm. *p < 0.05 and **p < 0.01

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Stem Cell Research & Therapy

ISSN: 1757-6512