Fig. 1

The effect of insulin on seeding of hiPSCs. hiPSCs were harvested with EDTA as cell aggregates and were imaged 1 day after seeding. a Whole-well images and microscopic images from the center of each well after crystal violet staining. b Quantification of the percentage surface area covered by hiPSC fragments for the microscopic images from a. Data represented as mean ± SD. Two-tailed Student’s t test was used for comparison of groups, each cell line in triplicates. hiPSC.3 colonies were harvested with accutase as single cells/small cell fragments and were passed through a 40-μm filter. c Whole-well images and microscopic images of the center of each well after crystal violet staining. d Quantification of percentage of surface area covered by hiPSCs for the microscopic images from c. Data represented as mean ± SD. One-way ANOVA test was used between first three groups; no adherent cells were detected in the last group. ***p < 0.001. ****p < 0001. ✢Significant difference between groups in LSD test. Experiment in triplicates. Scale bar: 2 mm. RI: rock inhibitor