Fig. 4

The effect of insulin on phosphorylation of RTKs, HPKs, and MAPKs. To investigate the effect of insulin on phosphorylation of target proteins in hiPSCs, the cells were cultured with or without insulin overnight followed by 15-min stimulation with insulin prior to harvesting the lysates for protein arrays. a Receptor tyrosine kinase (RTK) array. b Identified targets from RTK array and their respective quantification. c Human phosphokinase (HPK) array. d Identified targets from HPK array and their quantification. e Human phospho MAP kinase(MAPK) array. f Identified targets from MAPK array and their quantification