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Table 2 The parameters used to generate the experimental table. In total, 16 conditions were investigated to screen culture parameters for their effect on osteogenic differentiation. hASC human adipose-derived stromal cell, AP l-ascorbate-2-phospate, ßGP ß-glycerophosphate, DEX dexamethasone, BMP-9 bone morphogenetic protein-9. +, refer to the four added positive and negative controls

From: Design of experiments to assess the effect of culture parameters on the osteogenic differentiation of human adipose stromal cells

Condition hASC source Seeding density (cells/well) Medium compositions for osteogenic differentiation
Base medium Serum AP (μM) ßGP (mM) DEX (nM) BMP-9 (ng/mL)
1 EFS 10,000 DMEM/F-12 hPL 50 10 0 0
2 ZB 10,000 DMEM FBS 50 10 100 0
3 EFS 2000 DMEM hPL 0 10 100 100
4 ZB 10,000 DMEM/F-12 hPL 50 0 100 100
5 ZB 2000 DMEM FBS 0 10 0 100
6 ZB 2000 DMEM/F-12 hPL 0 10 100 0
7 EFS 2000 DMEM/F-12 FBS 50 0 100 100
8 EFS 10,000 DMEM/F-12 FBS 0 10 0 100
9 EFS 10,000 DMEM FBS 0 0 100 0
10 ZB 10,000 DMEM hPL 0 0 0 100
11 EFS 2000 DMEM hPL 50 0 0 0
12 ZB 2000 DMEM/F-12 FBS 0 0 0 0
13 ZB 6000 DMEM FBS 0 0 0 0
14+ ZB 6000 DMEM FBS 50 10 100 100
15 EFS 6000 DMEM FBS 0 0 0 0
16+ EFS 6000 DMEM FBS 50 10 100 100