Design of experiments to assess the effect of culture parameters on the osteogenic differentiation of human adipose stromal cells

Table 2 The parameters used to generate the experimental table. In total, 16 conditions were investigated to screen culture parameters for their effect on osteogenic differentiation. hASC human adipose-derived stromal cell, AP l-ascorbate-2-phospate, ßGP ß-glycerophosphate, DEX dexamethasone, BMP-9 bone morphogenetic protein-9. ⁺,⁻ refer to the four added positive and negative controls

Condition	hASC source	Seeding density (cells/well)	Medium compositions for osteogenic differentiation
Condition	hASC source	Seeding density (cells/well)	Base medium	Serum	AP (μM)	ßGP (mM)	DEX (nM)	BMP-9 (ng/mL)
1	EFS	10,000	DMEM/F-12	hPL	50	10	0	0
2	ZB	10,000	DMEM	FBS	50	10	100	0
3	EFS	2000	DMEM	hPL	0	10	100	100
4	ZB	10,000	DMEM/F-12	hPL	50	0	100	100
5	ZB	2000	DMEM	FBS	0	10	0	100
6	ZB	2000	DMEM/F-12	hPL	0	10	100	0
7	EFS	2000	DMEM/F-12	FBS	50	0	100	100
8	EFS	10,000	DMEM/F-12	FBS	0	10	0	100
9	EFS	10,000	DMEM	FBS	0	0	100	0
10	ZB	10,000	DMEM	hPL	0	0	0	100
11	EFS	2000	DMEM	hPL	50	0	0	0
12	ZB	2000	DMEM/F-12	FBS	0	0	0	0
13⁻	ZB	6000	DMEM	FBS	0	0	0	0
14⁺	ZB	6000	DMEM	FBS	50	10	100	100
15⁻	EFS	6000	DMEM	FBS	0	0	0	0
16⁺	EFS	6000	DMEM	FBS	50	10	100	100

ISSN: 1757-6512