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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Long-term culture of mesenchymal stem cells impairs ATM-dependent recognition of DNA breaks and increases genetic instability

Fig. 1

Induction of γH2AX- and 53BP1-foci formation after exposure to irradiation in aging MSCs in vitro. a MSCs were cultured for 1, 4, and 8 weeks in vitro. Repair foci formation is shown in MSCs 90 min after sham-irradiation (0 Gy) or after 2 Gy of γ-irradiation by immunofluorescence staining for γH2AX (red) and 53BP1 (green). Nuclear counterstaining was done using DAPI. b Quantification γH2AX- and 53BP1-foci formation in MSCs was done 90 min after sham-irradiation (0 Gy) or after γ-irradiation with 0.05, 0.5, 2, and 6 Gy using the Keyence BZ-II Analyzer software (see Additional file 6: Figure S1). Mean number of foci ± SEM (n = 3) are calculated from at least 50 analyzed nuclei

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Stem Cell Research & Therapy

ISSN: 1757-6512