Fig. 2

The properties of passage 1 MEPM cells. a Immunofluorescence staining of MEPM cells for vimentin (green), nestin (red), HNK-1 (green) (white arrow), and keratin (not stained). b Immunofluorescence staining of MEPM cells for PDGFRα (green), Efnb1 (red), Osr2 (red), and Meox2 (red). c Transwell assays showing that PDGFA (0.5 ng/mL and 5 ng/mL) stimulates chemotaxis of MEPMs. N = 9; *P < 0.05; **P < 0.01. Cell nuclei (blue) were counterstained with DAPI, and HNK-1 (red) in cytoplasm was stained with Evans blue dye. Scale bar: 20 μm