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Table 1 Primer sequences for RT-qPCR

From: Exosomes of stem cells from human exfoliated deciduous teeth as an anti-inflammatory agent in temporomandibular joint chondrocytes via miR-100-5p/mTOR

Gene

Forward primer (5′–3′)

Reverse primer (5′–3′)

IL-6

AGCCCACCGGGAACGA

GGACCGAAGGCGCTTGT

IL-8

AGAAGTTTTTGAAGAGGGCTGAGA

AGTTTCACTGGCATCTTCACTGATT

MMP1

ACTGCCAAATGGGCTTGAAG

TTCCCTTTGAAAAACCGGACTT

MMP3

GAGGCATCCACACCCTAGGTT

TCAGAAATGGCTGCATCGATT

MMP9

CCCTTGTGCTCTTCCCTGGA

TCTGCCACCCGAGTGTAACC

MMP13

ATTAAGGAGCATGGCGACTTCT

CCCAGGAGGAAAAGCATGAG

ADAMTS5

GGCCTCCATCGCCAATAGG

GGATAGCTGCATCGTAGTGCT

miR-100

GTGTTCAAGCCTAGATGCCCAA

GCATCTAGGCTTGAACACGCC

mTOR

TCTTTCATTGGAGACGGTTTGG

AGGTTTTCATGGGATGTCGCT

U6

CGCTTCGGCAGCACATATAC

AAATATGGAACGCTTCACGA

GAPDH

CTTTGGTATCGTGGAAGGACTC

GTAGAGGCAGGGATGATGTTCT

  1. IL-6 Interleukin-6, IL-8 Interleukin-8, MMP1 matrix metalloproteinase-1, MMP3 matrix metalloproteinase-3, MMP-9 matrix metalloproteinase-9, MMP13 matrix metalloproteinase-13, ADAMTS5 disintegrin and metalloproteinase with thrombospondin motifs 5, miR-100 microRNA-100, mTOR mammalian target of rapamycin, GAPDH glyceraldehyde 3 phosphate dehydrogenase, RT-qPCR Reverse transcription-quantitative polymerase chain reaction
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Stem Cell Research & Therapy

ISSN: 1757-6512