Fig. 7

IL-10-mediated immune suppression of NVs in OMV-induced sepsis. a RAW 264.7 cells were pre-incubated with OMVs (100 ng/mL) for 3 h and treated with various doses of NVs for 15 h, and the concentration of IL-10 in the conditioned media was measured. n = 3/group. b, c The concentration of IL-10 was evaluated in the peritoneal fluid (b) and serum (c) at 6 h following OMVs administration. n = 10/group. d, e mIL-10 or isotype-matched control antibody was intraperitoneally injected together with OMVs, followed by injection NVs after 1 h. Serum concentrations of TNF-α (d) and IL-6 (e) were measured at 6 h. n = 5/group. f The percentage of T regulatory cells in the peritoneum were determined by FACS analysis at 6 h. n = 5/group. *P < 0.05; **P < 0.01; ***P < 0.001; ns, not significant. Error bars indicate SEM