Fig. 5
From: Isolation and characterization of a new basal-like luminal progenitor in human breast tissue
BLPs lose CD90 expression during differentiation. a Cell sorting strategy to isolate the basal-like luminal progenitors (BLPs, NOTCH3+FZD7+) and the NOTCH3+FZD7+ subset of the luminal-restricted progenitors (LRPs) from precultured breast reduction samples that are placed in colony assays. Bar graphs show the average ± SD mean fluorescence intensity of NOTCH3 (b) and FZD7 (c) expression in BLPs and LRPs. d Bar graphs show the average ± SD progenitor frequency in subsets “a” and “b” of LRPs. e Representative immunofluorescent staining of colonies generated from BLPs and LRP-subset a for cytokeratin 8/18 (green), DAPI (blue), and CD90 (red). The lines represent 1000 μm. All data obtained from 3 independent breast reduction samples. Additional file 9: Figure S5A shows mix colonies stain positive for CD90 expression (***p ≤ 0.005)