Fig. 6
From: Isolation and characterization of a new basal-like luminal progenitor in human breast tissue
BLPs differ from LRPs in their ERα and MUC1 expression. a The bipotent progenitors (BPs), basal-like luminal progenitors (BLPs), and the luminal-restricted progenitors (LRPs) were obtained from precultured breast reduction samples, fixed on glass slides, and stained with fluorescent antibodies to detect estrogen receptor alpha (ERα) expression. Representative images of ERα expression in the different progenitor subsets are shown (the line represent 200 μm) and the average frequency ± SD of ERα+ cells in each progenitor subset from 4 independent breast reduction samples are shown in bar graphs (b). LRPs and BLPs were placed in colony assay and the colonies were stained with (c) CK8/18 (red), ERα (green), and DAPI (blue) (c) or (d) MUC1. The line represents 400 μm