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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Modulation of osmotic stress-induced TRPV1 expression rescues human iPSC-derived retinal ganglion cells through PKA

Fig. 1

Generation of hiPSC-derived RGCs. a Schematic of the protocol for differentiating hiPSCs into RGCs. Culturing conditions (suspension or adherent) are shown at the top in green, crucial morphogenic factors shown at the top in blue, the intermediate stages EB—embryoid bodies, NR—neural rosettes, and OV—optic vesicles. b Bright-field images of RGCs after dissociation from optic vesicles from day 1 to day 21. c Flow cytometry analysis of the proportion of Thy-1-expressing cells in RGC populations differentiated by original and modified methods. d Western blot analysis demonstrating expression of positive RGC markers in mature day 21 hiPSC-derived RGCs as compared to neural rosette (NR) stage. e Expression of positive RGC markers in the mature day 21 RGCs demonstrated by immunofluorescent staining. f Electrophysiological analysis of the mature day 21 RGCs (bottom) compared to immature day 21 RGCs (top)

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Stem Cell Research & Therapy

ISSN: 1757-6512