Fig. 1
Evaluation of MSC classical markers at 30 days post-differentiation. After the induction protocol, the expression of MSC markers were evaluated by FACS. a CD105 is lost in PreMel after differentiation and reaches similar levels to HEM, dot plot shows the percentage of positive cells. b MFI ratio of CD105 in ADSC, PreMel, and HEM. c Dot plots shows the high percentage of CD73-positive cells in ADSC, PreMel, and HEM. d MFI ratio shows that CD73 expression decreases by 50% in PreMel compared to ADSC. e Distribution of CD105 and CD73 expression in ADSC, PreMel, and HEM. f Percentage of CD105− CD73low cells in ADSC, PreMel, and HEM. MFI ratio was calculated as MFI (specific staining)/MFI (autofluorescence) for each marker. Data from PreMel and HEM were normalized with respect to undifferentiated ADSC. For dot plots, gray dots represent autofluorescence control, and black dots represent stained cells with their respective marker. n = 3, *P < 0.05 one-way ANOVA