Fig. 2
From: Human amniotic epithelial cells improve fertility in an intrauterine adhesion mouse model
Endometrial morphology in hAECs was restored in a mouse model of intrauterine adhesions. A (a) H&E staining showed that the endometrium of a normal mouse was in a contact arrangement, with abundantly scattered glands and columnar epithelium cells lined clearly on the cavity surface. (b) In the IUA model, the uterine cavity was adherent. (c) In the hAEC-treated group, uterine adhesion decreased. Scale bar = 200 μm. B Compared with that in the control group, the number of glands decreased in the IUA group, but the hAEC-treated group showed a higher number of glands than the IUA group. C The thickness of the endometrium in the IUA group was decreased compared to that in the control group and hAEC-treated group. D A larger percent of the fibrosis area of the endometrium was found in the IUA model. The fibrosis area decreased in the hAEC-treated group. E Masson staining showed fibrotic tissue (stained pale blue) in the endometrium. A fibrotic mass was found in the damaged endometrium (black arrow pointing) (b, e, h), and the fibrosis area was larger in the IUA group than in the control group (a, d, g) and hAEC-treated group (c, f, i). a–c, scale bar = 200 μm; d–f, scale bar = 100 μm; g–i, scale bar = 25 μm (n = 6; *p < 0.05; **p < 0.01; ***p < 0.001; NS, p ≥ 0.05)