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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Genotyping, generation and proteomic profiling of the first human autosomal dominant osteopetrosis type II-specific induced pluripotent stem cells

Fig. 4

Detection of gene expression by PCR. a The picked ADO2-iPSCs on day 17 after plasmid infection (passage number = 0, P0) are positively expressed with SeV genome and transgenes; the passage ADO2-iPSCs ((passage number = 10, P10) are similar to the H9 cell lines, and they are negative for the SeV genome and transgenes. b, c EB formation was generated from the ADO2-iPSCs, and the marker genes, including GATA4, SOX1, and MSX1, were positively expressed. The grouping of gels was cropped from different parts of the same gel and from the full-length agarose gel, which are presented in Additional file 1: Figure S1. d Teratoma formation in the backs of NOD-SCID mice after 8 weeks of the injection with ADO2-iPSCs. e The teratomas taken from the mice. f Typical histology of the teratomas with all three germ layers (endoderm, mesoderm, and ectoderm)

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