Fig. 1

a The experimental timeline of oligodendrocyte differentiation from hiPSC colonies. b Phase bright images of iPSC colonies, neurorossette formation on day 5, oligospheres in suspension cultures, and O4+ Pre-OLs. c Expression pattern of SHH pathway genes in undifferentiated hiPSC, hiPSC-derived NSCs, and OLIG2-OPCs (three independent RT-PCR assays, n = 3, t test). d The generation OLIG2+/GFP+ glial progenitors (on day 14) following purmorphamine treatment in NSCs from day 6 to 14 (top row) and the absence of OLIG2/GFP induction without purmorphamine (second row); GANT61 treatment in NSC concomitant with purmorphamine (from day 6 to 14) did not give rise to OLIG2/GFP glial progenitors (third row), and GANT61 treatment from day 0 to 5 followed by purmorphamine (days 6–14) gave rise to OLIG2+/GFP+ glial progenitors (fourth row). The images in d is a representation of three independent experiments. Scale bar, 100 μm. Error bars indicate SEM. ***P < 0.001; **P < 0.01; *P < 0.05 vs. day 0