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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Chrysosplenetin promotes osteoblastogenesis of bone marrow stromal cells via Wnt/β-catenin pathway and enhances osteogenesis in estrogen deficiency-induced bone loss

Fig. 6

The effect of Chrysosplenetin on total and phosphorylation of β-catenin. Protein lysates from OIM-induced hBMSCs were pre-treated with Chrysosplenetin (10 μM), which were added with Noggin (10 μg/ml) or DKK1 (0.5 μg/ml) at days 3, 7, and 14. The protein expressions of β-catenin (a) and p-β-catenin (b) were tested by Western blot assay with specific antibodies. Relative results were expressed by the ratio of the amount of β-catenin/β-actin (c) and p-β-catenin/β-actin (d) determined by Image J (*p < 0.05, **p < 0.01, ***p < 0.001 relative to Chrysosplenetin-treated group)

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