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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: PBX homeobox 1 enhances hair follicle mesenchymal stem cell proliferation and reprogramming through activation of the AKT/glycogen synthase kinase signaling pathway and suppression of apoptosis

Fig. 6

PBX1 activated the ATK/GSK3β signaling pathway in induced pluripotent stem cells. a, b Transduced HF-MSCs were harvested on day 7 to assess the levels of phospho-AKT, phospho-GSK3β, p16, p21, and β-catenin. GAPDH and HISTONE were used as loading controls. The value in SOMK-transduced HF-MSCs as the control group was set as 1.0. c Immunofluorescence analysis of β-catenin and p53 expression and localization in transduced HF-MSCs (bar, 100 μm). d, e Transduced HF-MSCs were harvested on day 21 to assess the levels of phospho-AKT, phospho-GSK3β, p16, p21, NANOG, and β-catenin. GAPDH and HISTONE were used as endogenous controls for equal loading. The value in SOMK-transduced HF-MSCs as the control group was set as 1.0. *P < 0.05; **P < 0.01; ***P < 0.001

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Stem Cell Research & Therapy

ISSN: 1757-6512