Table 1 Primers for qPCR
Gene | Forward primers (5′ to 3′) | Revers primers(5′ to 3′) |
|---|---|---|
PBX1 | GAGACGGAATTTCAACAAGCA | GTTTGATACCTGGGAGACTG |
Endo-OCT4 | GGGAGGAGCTAGGGAAAGAAAACCT | GAACTTCACCTTCCCTCCAACCAGT |
Endo-SOX2 | TTAGAGCTAGTCTCCAAGCGACGA | CCACAGAGATGGTTCGCCAG |
NANOG | ATGGAGGGTGGAGTATGGTTGG | AGGCTGAGGCAGGAGAATGG |
CRIPTO | TACCTGGCCTTCAGAGATGACA | CCAGCATTTACACAGGGAACAC |
FOXD3 | AAGCCCAAGAACAGCCTAGTGA | GGGTCCAGGGTCCAGTAGTTG |
LIN28 | CAGGTGCTACAACTGTGGAGG | GCACCCTATTCCCACTTTCTCC |
FGF4 | CTACAACGCCTACGAGTCCTACA | GTTGCACCAGAAAAGTCAGAGTTG |
ESG1 | ATATCCCGCCGTGGGTGAAAGTTC | ACTCAGCCATGGACTGGAGCATCC |
GAPDH | CCATGTTCGTCATGGGTGTGA | CATGGACTGTGGTCATGAGT |