Fig. 4

TGF-β1 induces Procol1A1 and α-SMA expression by MRTF-A/SRF-dependent signaling in HIMFs. HIMFs were pretreated with or without CCG-100602 (40 μmol/L) for 30 min prior to the addition of TGF-β1 (5 ng/mL) for 48 h and then stained with Procol1A1, α-SMA, and MRTF-A antibodies and rhodamine-phalloidin and counterstained with Hoechst. a α-SMA. b Procol1A1. c MRTF-A and F-actin. d The nuclear-to-cytoplasmic ratio of MRTF-A was determined for each condition as described in the the “Methods” section. Data are expressed as the means ± SEM. *P < 0.05 versus the TGF-β1 treatment only. NS, not significant. Scale bars, 100 μm; original magnification, × 200 (a–c)