Fig. 5
Type 3 NFC dressing did not alter the undifferentiated state of hASCs. a Quantitative PCR for hASCs cultured on type 3 NFC dressing for 7 days. Relative expressions normalized to the expression of endogenous control gene β-2-m for hASCs cultured with 300,000 cells/cm2 (300k) cell density with (NFC 300k) or without (300k) type 3 NFC dressing for 7 days showing no statistical difference in the expression of specific cell surface antigens CD73, CD90, CD105, CD166, or CD34; cell cycle marker CCND1; stemness markers OCT4, SOX2, and NANOG; or differentiation markers PPARγ, RUNX2, or COL2A1 compared with 30k cell density. Ctrl 30k and 300k n = 4, NFC 300k n = 3. b Reverse transcriptase PCR. Negative expression of a specific cell surface antigen CD45 was observed for hASCs regardless of the culture conditions. β-2-m, beta-2-microglobulin; NFC, nanofibrillar cellulose; NTC, non-template control