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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Mechanical process prior to cryopreservation of lipoaspirates maintains extracellular matrix integrity and cell viability: evaluation of the retention and regenerative potential of cryopreserved fat-derived product after fat grafting

Fig. 2

Freeze-induced tissue injury. a TUNEL assay of tissue cryosections. Frozen sections of fresh fat, fresh SVF gel, and 1- or 3-month cryopreserved fat and SVF gel were subjected to the TUNEL assay to measure apoptosis (TUNEL+ cells). Prolonged cryopreservation without heat recovery exacerbated SVF cell apoptosis. The rate of SVF cell death was 36 ± 1% at 1 month and 53 ± 3% after 3 months of freezing. **p < 0.01. Scale bar = 50 μm. b Flow cytometry density plot of 7-AAD staining for SVF cells. SVF cells collected by enzymatic digestion were analyzed by flow cytometry. Non-apoptotic cells were 7-AAD-negative. The 7-AAD (−) population is enclosed within the green dash line. The percentage of non-apoptotic cells was more than twofold greater in cryo-gel (56 ± 7%) than in cryo-fat (25 ± 1%). *p < 0.05. n = 3 independent experiments with 3 donors

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Stem Cell Research & Therapy

ISSN: 1757-6512