Table 1 Specific primers used for RT-PCR
From: Osteogenic potential of induced pluripotent stem cells from human adipose-derived stem cells
Gene | Forward | Reverse |
|---|---|---|
GAPDH | 5′-ATGGGGAAGGTGAAGGTCG-3′ | 5′-GGGGTCATTGATGGCAACAATA-3′ |
Col I | 5′-CCGCCGCTTCACCTACAGC-3′ | 5′-TTTTGTATTCAATCACTGTCTT-3′ |
OCN | 5′-AGCAAAGGTGCAGCCTTTGT-3′ | 5′-GCGCCTGGGTCTCTTCACT-3′ |
ALP | 5′-GTTCAGCTCGTACTGCATGTC-3′ | 5′-ATCGCCTACCAGCTCATGCAT-3′ |
RunX-2 | 5′-ATTCCTGTAGATCCGAGCACC-3′ | 5′-GCTCACGTCGCTCATTTTGC-3′ |