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Table 1 Specific primers used for RT-PCR

From: Osteogenic potential of induced pluripotent stem cells from human adipose-derived stem cells

Gene Forward Reverse
GAPDH 5′-ATGGGGAAGGTGAAGGTCG-3′ 5′-GGGGTCATTGATGGCAACAATA-3′
Col I 5′-CCGCCGCTTCACCTACAGC-3′ 5′-TTTTGTATTCAATCACTGTCTT-3′
OCN 5′-AGCAAAGGTGCAGCCTTTGT-3′ 5′-GCGCCTGGGTCTCTTCACT-3′
ALP 5′-GTTCAGCTCGTACTGCATGTC-3′ 5′-ATCGCCTACCAGCTCATGCAT-3′
RunX-2 5′-ATTCCTGTAGATCCGAGCACC-3′ 5′-GCTCACGTCGCTCATTTTGC-3′
  1. GAPDH glyceraldehyde-3-phosphate dehydrogenase, Col I collagen type I, OCN osteocalcin, ALP alkaline phosphate, RunX-2 runt-related transcription factor 2