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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Direct conversion of human fibroblast to hepatocytes using a single inducible polycistronic vector

Fig. 2

Generation of human iHEP using a single polycistronic lentivirus. a Morphological changes after HDF and HDF-LT reprogramming to iHEP (see Fig. 1b for details). b DOX treatment halted cell proliferation. HDF-LTDOX were treated with 1000 ng/mL DOX and cells were counted by cell cytometry at different time points. c HDF-LTDOX were treated with 1000 ng/mL DOX and RNA isolated at multiple time points. ALB mRNA expression was quantified by qRT-PCR and expressed relative to the expression at 10 days. d, e HDF-LTDOX were treated with different concentrations of DOX and RNA isolated after 10 days. ALB and exogenous transgene expression was quantified by qRT-PCR and expressed relative to iHEP-LT levels (see Fig. 1b for details)

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