Fig. 4
Artemisinin attenuated H2O2-induced ΔΨm reduction. BMSCs were pretreated with 1.0 μM artemisinin or DMSO (0.1%, vehicle control) for 1 h, then exposed to 200 μM H2O2 for another 24 h. a ΔΨm was analyzed using JC-1 assay (n = 3; scale, 100 μm). The decline of the membrane potential was reflected by the shift of fluorescence from red to green indicated by JC-1. b The quantitative data of the red/green ratio (n = 3). ***p < 0.005. CTRL, control; ART, artemisinin; H2O2, exposed to hydrogen peroxide only; ART + H2O2, treated with artemisinin followed by exposure to hydrogen peroxide