Fig. 2

Effects of BMSC supernatant treatment on demyelination of the spinal cord and brain. a Demyelination for lumbar spinal cord sections at peak phase by Luxol fast blue staining, magnification × 100. In brain sections, cell infiltration was observed in the injured area of EAE mice (indicate by red arrow), c CD45R-positive B cells, e CD68-positive mononuclear cells, g CD4-positive T cells, i CD86-positive antigen presenting cells, and k Iba-1-positive and m P2ry12-positive microglia, magnification × 200. Percentage area of demyelination in the spinal cord white matter (b) and the number of positive cells per mm² (d, f, h, j, l, n) calculated by analysis of transverse sections at three separate levels per mouse. Data represent the mean ± SEM of three individual mice per group, **P < 0.01, ***P < 0.001