Fig. 4

Effect of BMSC supernatant treatment on B cell function. We took B lymphocytes (CD19⁺) from the spleen at the peak phase of EAE mice and cultured them in vitro with BMSC supernatant or stem cell-specific medium as control. a Proportion of GM-CSF producing B cells and IL-10 producing B cells (gated in CD19⁺ B cells) after in vitro treatment of BMSC supernatant. b Statistical analysis of the ratio of GM-CSF producing B cells and IL-10 producing B cells after in vitro treatment of BMSC supernatant, mean ± SEM, n = 5, ***P < 0.001. c After BMSC supernatant treatment, the cell culture supernatant was collected, and the secretion of antibodies by B cells in the cell culture supernatant was detected by ELISA, mean ± SEM, n = 5, *P < 0.05, ***P < 0.001. d Secretion of cytokines GM-CSF, IFN-γ, TNF-α, and IL-1β of B cells after BMSC supernatant treatment, mean ± SEM, n = 5, *P < 0.05, ***P < 0.001