Fig. 4
From: Supplementation of vitamin C promotes early germ cell specification from human embryonic stem cells
Epigenetic analyses of PGCLC induction stimulated by vitamin C. a, b Global 5hmC and 5mC levels detected by dot blot in different concentrations of vitamin C (0, 50, 100, 200 μg/ml); n = 3 independent experiments. c Analyses of 5hmC levels by ELISA; n = 3 independent experiments. Data are presented as mean ± SD. Statistical analysis was performed by one-way analysis of variance. *p < 0.05. d Expression analysis of epigenetic regulators by RT-qPCR for day 4 EBs stimulated by different of concentrations of vitamin C (0, 50, 100, 200 μg/ml). Relative expression levels are shown with normalization to hESCs; n = 3 independent experiments. Data are presented as mean ± SD. Statistical analysis was performed by one-way analysis of variance. *p < 0.05