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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Extracellular vesicles derived from human Wharton’s jelly mesenchymal stem cells protect hippocampal neurons from oxidative stress and synapse damage induced by amyloid-β oligomers

Fig. 6

Catalase mediates the ability of hMSC-EVs to prevent synapse damage induced by AβOs. a, b Representative images from cultured hippocampal neurons exposed to 500 nM AβOs or vehicle for 24 h and immunolabeled for synaptophysin (SYP, green) and PSD-95 (red). c, d Where indicated, hippocampal cultures were treated with hMSC-EVs or hMSC-iEVs. e, f Representative images from vehicle-exposed cultures treated with hMSC-EVs or hMSC-iEVs. Integrated fluorescence intensities for synaptophysin (g) or PSD-95 (h), and numbers of co-localized synaptophysin/PSD-95 punctae (i). Bars represent means ± SEM from three experiments using independent cultures. Nine to 12 images from each of three coverslips were acquired and analyzed in each independent experiment. *p < 0.05; **p < 0.01; ***p < 0.001; one-way ANOVA followed by Tukey’s post hoc test. Scale bar, 20 μm

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Stem Cell Research & Therapy

ISSN: 1757-6512