Fig. 5

Migratory capacity of CD51⁻bMSCs and CD51⁺bMSCs in vitro. a, b Images of scratch-wound cell migration assays at 0 h and 24 h; the relative repair area was determined by the total area of migrated cells in the wounds (n = 6). c, d Transwell assay; the migratory cells were fixed at 8 h after plating and stained with crystal violet. Bright-field colour images and fluorescence images were randomly taken, and the cell numbers were calculated (n = 8). e, f Relative mRNA expression of the chemokine receptors; the gene expression levels were normalized to GAPDH (n = 3). Data in all panels are presented as the mean ± SD, **P < 0.01, **P < 0.001. Scale bars are marked in the figure