Fig. 4

Sox9 and Runx2 were modified by O-GlcNAc. CESCs were induced under low-glucose (LG, 1 mM) conditions in chondrogenic induction medium (CIM) (a) or osteogenic induction medium (OIM) (b), respectively, for 21 days. a Immunofluorescence staining of CESCs induced in chondrogenic induction medium for 21 days. b Immunofluorescence staining of CESCs induced in osteogenic induction medium for 21 days. c, d Immunoprecipitation of Sox9 and Runx2 in CESCs induced in CIM and OIM