Fig. 4

High concentration of silicate did not affect lysosome function of BMSCs. a, b Lyso-tracker assay, which could lock acid lysosomes, was applied to evaluate the function of lysosomes, and the Baf A1 and CQ treatment groups were set as the positive control groups (n = 3, 30 random cells per sample). The data indicated that the fluorescence intensity in the silicate (3 mM)-treated groups was similar to that of the CQ-treated group, and the intensity increased significantly compared with that of the control group. c, d Western blot was performed to characterize the expression of LAMP1 and LAMP2 in BMSCs after exposure to silicate for 24 h (n = 4). Data analysis showed that LAMP1 and LAMP2 increased significantly in the high-concentration silicate group. (*p < 0.05, **p < 0.01, ***p < 0.001)