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Fig. 7 | Stem Cell Research & Therapy

Fig. 7

From: Microtubule destabilization caused by silicate via HDAC6 activation contributes to autophagic dysfunction in bone mesenchymal stem cells

Fig. 7

Inhibition of HDAC6 activity restored microtubule stability after silicate exposure. HDAC6 expression was silenced via siHDAC6 transfection (50 nM) and subsequently exposed to a high dose of silicate (3 mM). a, b, c HDAC6 expression and activity were significantly downregulated compared with that of the control group after transfection with siHDAC6 from Western blot detection and HDAC6 activity assay, and the suppression of HDAC6 significantly increased the expression of acetyl-α-tubulin with Western blot detection (n = 4). d Collapsed state and bundle or reticular structure of microtubules, caused by high-dose silicate, was partially restored via the suppression of HDAC6. (**p < 0.01, ***p < 0.001)

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Stem Cell Research & Therapy

ISSN: 1757-6512