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Fig. 8 | Stem Cell Research & Therapy

Fig. 8

From: Microtubule destabilization caused by silicate via HDAC6 activation contributes to autophagic dysfunction in bone mesenchymal stem cells

Fig. 8

Inhibition of HDAC6 activity restored autophagic activity and improved cell viability after silicate exposure. a, b Autophagic flux-related proteins including p62 and LC3-II were identified by Western blot after transfection with siHDAC6 (n = 4). Data analysis showed that the expression of p62 and LC3-II after high-dose silicate treatment was partially downregulated via HDAC6 interference compared with that of the control group. c, d Autophagosome (LC3 dots) accumulation caused by high-dose silicate was partially improved with siHDAC6 treatment (n = 3, 10 random fields per sample). e CCK-8 assay was applied to evaluate cell viability after siHADC6 treatment. As the data show, the downregulation of cell viability caused by 1.5 mM and 3 mM silicate was significantly improved after siHDAC6 treatment (n = 4). (*p < 0.05, **p < 0.01, ***p < 0.001)

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Stem Cell Research & Therapy

ISSN: 1757-6512