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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: MSC-secreted TGF-β regulates lipopolysaccharide-stimulated macrophage M2-like polarization via the Akt/FoxO1 pathway

Fig. 1

LPS-induced inflammatory reaction and M1-like properties macrophages. RAW264.7 cells were stimulated with or without LPS for 12, 24, 48, and 72 h. a The effect of different concentrations of LPS on cell viability in RAW264.7 cells at 24 h, as tested by CCK8. b The effect of LPS (500 ng/ml) on IL-6 and IL-1β production, as tested by ELISA. c Characteristics of mouse peritoneal macrophages by flow cytometry and immunofluorescence. d The effect of LPS (500 ng/ml) on MFI of CD86, as tested by flow cytometry. *p < 0.05 vs. control group; **p < 0.05 vs. control group; ***p < 0.001 vs. control group. Data show the mean ± SD (n = 3). APC, allophycocyanin; ELISA, enzyme-linked immunosorbent assay; IL-6: interleukin 6; IL-1β: interleukin 1β; LPS: lipopolysaccharide; MFI, mean fluorescence intensity; PMA: peritoneal macrophages

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Stem Cell Research & Therapy

ISSN: 1757-6512