Fig. 2

Acetylation of histone H2B and chromatin state under fluid shear. Histone acetylation was shown in (a) with typical immunofluorescent images of H2B, AcH2B, and DNA (left three columns) as well as the threshold transformation of AcH2B (right column; corresponding to the respective yellow boxes in the 2nd column from the left). Also plotted were the measured fluorescence intensities corresponding to AcH2B/DNA ratio (b) and the mean AcH2B and DNA intensities (c). The numbers of tested replicates and measured colonies, (M, m), are (4, 49) for control, (3, 65) for fluid shear, and (3, 34) for TSA treatment. Flow direction was indicated by solid arrows in a. Bar = 20 μm in a. Here Shear denotes the steady shear flow of 1.1 Pa for 24 h