Fig. 5

Colony polarity and H2B acetylation. Microscopic images of a typical colony were illustrated under static control or fluid shear (a), and the relevant analysis protocol was given for pseudopodium number and perimeter (b). Also plotted were the pseudopodia number per unit contour length (c) and total pseudopodia perimeter normalized by colony contour length (d) under static control or fluid shear, as well as the localized expression of H2B acetylation at the edge under fluid shear (e). Pseudopodia were shown in the red circles in a. Five colonies were measured under each condition. Bar = 100 μm in a and 50 μm in e. Here Shear denotes the steady shear of 1.1 Pa for 24 h