Fig. 1

Analysis of ETV2-regualted miRNA expression in FLK1⁺ cells. a Schematic diagram of miRNA sequencing experiment. Doxycycline-inducible (iFLAG-ETV2) mESCs were differentiated, treated with ± Doxycycline (Dox) at day 1 and sorted for FLK1⁺ cells at day 3.5. RNAs from the sorted cells were subjected to miRNA sequencing and analysis. b Volcano plot showing the log2 fold change between +Dox versus −Dox against the −log10 FDR-adjusted p value for each miRNA. miRNAs (FDR ≤ 0.05) with fold change of ≥ 1.5 (in red; upregulated) and ≤ − 1.5 (in green; downregulated) were highlighted and selected. c Heatmap of the selected miRNAs in response to overexpression of ETV2. miRNAs upregulated and downregulated in +DOX were indicated with gray and black bars, respectively. d Gene Ontology (GO) categories of selected miRNAs by DIANA miRpath analysis. Bars indicate the significance level of miRNA target genes and interactions. e Differentiated iFLAG-ETV2 mESCs at day 3.5 were subjected to qRT-PCR analysis. n = 3, ***p < 0.001