Fig. 2
NADHhigh glioma cells exhibit characteristics of GSCs in vitro. a qRT-RCR and western blotting analyses showed higher expression levels of stem-related genes in NADHhigh cells than in NADHlow cells in GBM1 and LN229 cell lines. b, c Limiting dilution assays showed higher tumorsphere formation rates and longer average diameter of tumorsphere in NADHhigh cells compared to NADHlow cells in GBM1 and LN229 cell lines. d Cultured in medium supplemented with 10% FBS for 7 days, NADHhigh cells markedly reduced the autofluorescence of NADH and the expression of neural stem/progenitor markers Sox2 and Nestin, but re-expressed high astroglial marker GFAP. Scale bar = 50 μm. All data are presented as the means ± SD. *p < 0.05, **p < 0.01, ***p < 0.001 (n = 3 independent experiments)