Fig. 3
MVs derived from AZA/RES-treated ASC enhance ASCEMS proliferation. Different dosages of MVs were added to ASC in order to investigate whether MVs work in a dose-dependent manner (a). The first measurement was performed after 24 h of co-culture. After that time, the medium was exchanged and cell proliferation was monitored till 96 h of culture. For further experiments, cells were cultured for 24 with 25 μg/ml of MVs. BrdU analysis confirmed that selected concentration enhances cellular proliferation. Results are expressed as mean ± SD, *p < 0.05; ***p < 0.001