Fig. 4

Hematopoietic supporting effect of hUC-MSCs at various passages in vitro. a hUC-MSCs at various passages were cultured with PBMC. The inhibition of lymphopoiesis or specific sub-lymphocyte proliferation (Th1, Th2, Treg, Th17) was normalized to the level (= 1) of those in the Ctr group. Data shown as mean ± SEM (n = 3). *P < 0.05; **P < 0.01; ***P < 0.001; NS, not significant. b, c hUC-MSCs at various passages were cultured in 10% FBS/IMDM medium for 72 h; the supernatant was collected and cocultured with PBMCs for 14 days. The total colony number, G colony number, M colony number, GM colony number, and total PBMC number were counted and calculated. d Flow cytometry analysis of the cocultured PBMC-derived cell population with indicated antibodies (CD19, CD11b, CD34, CD3, CD14, CD45, CD66b). All values normalized to level (= 1) of those in the Ctr group. Data shown as mean ± SEM (n = 3). *P < 0.05; **P < 0.01; ***P < 0.001; NS, not significant