Fig. 1

Metformin treatment attenuates muscle wasting in severely burned mice. a The percentage (%) body weight change in sham, burn, and burn + metformin at 7 days post-burn in mice. b Gastrocnemius muscle mass expressed as a dry/wet muscle ratio in sham, burn, and burn + metformin at 7 days post-burn in mice. c Representative images of hematoxylin and eosin staining of the gastrocnemius muscle from sham, burn, and burn + metformin mice at 2 and 7 days post-burn injury. Myofibers are significantly smaller at 7 days post-burn injury. Metformin treatment restores myofiber size. Images were taken at × 20 magnification. d Quantification of muscle cross-sectional area (μm²) in sham, burn, and burn + metformin at 2 and 7 days post-burn injury