Table 2 Survival status and progeny statistics of C57BL/6 mice after transplantation with SSCs from each of the three purification methods (mean ± SD)
Group (C57BL/6 group) | Density gradient centrifugation group (n = 10) | Flow cytometry sorting group (n = 10) | Immunomagnetic bead sorting group (n = 10) |
|---|---|---|---|
Number of cases of leukemia | 10 (100%) | 0 | 0 |
Average number of days of leukemia | 18.2 ± 3.6 | – | – |
Average survival time | 30.9 ± 4.8 | 113.1 ± 12.2 | 114.4 ± 11.3 |
Number of cases of testicular GFP sperm detected | 0 | 10 (100%) | 10 (100%) |
Average detection time of testicular GFP sperm | – | 50.1 ± 4.6 | 42.8 ± 6.0 |
Number of births of offspring GFP mice | 0 | 8 (80%) | 10 (100%) |
Number of births of offspring non-GFP mice | 0 | 0 (0%) | 0 (0%) |