Table 1 Proteomic studies of MSC-EVs
From: Functional proteins of mesenchymal stem cell-derived extracellular vesicles
References | Sources of MSCs and MSC-EVs | Analytic techniques | Major findings |
|---|---|---|---|
Kim et al. [42] | Human BM-derived MSC-EVs | LC-MS/MS | 730 proteins including markers of MSCs and EVs as well as proteins involved in the therapeutic effects of MSCs |
Angulski et al. [43] | Human BM-derived MSC-EVs | Nano LC-MS/MS | 797 proteins with 60% overlapping with those of Kim et al. |
Anderson et al. [44] | Human BM-derived MSC-EVs | Nano LC-MS/MS | Total 1927 proteins under normoxic and hypoxic conditions, with increased expression of angiogenic proteins under hypoxic condition |
La Greca et al. [45] | Human pluripotent stem cell-derived MSC-EVs | LC-MS/MS | 560 proteins enriched with immune, extracellular matrix, and cell adhesion molecules compared with MSCs |
Lai et al. [46] | Human embryonic stem cell-derived MSC-EVs | LC-MS/MS and antibody arrays | 766 proteins via MS analysis and 101 via antibody array, with functions such as communication, motility, inflammation, and biogenesis of EVs |
Lai et al. 2016 [47] | Human embryonic stem cell-derived MSC-EVs | LC MS/MS | 1806 proteins in CTB-bound fraction, 1547 proteins in CTB-depleted fraction, and 987 proteins in both fractions, with function of EV biogenesis in CTB-bound fraction |
Otero-Ortega et al. [48] | Rat adipose-derived MSC-EVs | LC-MS/MS | 2416 proteins, a big percentage of which was related to brain repair function |
Eirin et al. [49] | Pig adipose-derived MSC-EVs | LC-MS/MS | 4937 proteins with 128 enriched proteins, which were associated with tissue regeneration |
Eirin et al. [50] | Pig adipose-derived MSC-EVs | LC-MS/MS | 5623 proteins with 277 enriched proteins along with 4 enriched miRNAs and 255 enriched mRNAs in an integrated transcriptomic and proteomic analysis |
Eirin et al. [51] | Adipose-derived MSC-EVs from pigs with metabolic syndrome or control | LC-MS/MS | 6690 proteins with 146 enriched proteins relating regeneration in control EVs. 6790 proteins with 787 enriched proteins relating pro-inflammatory pathways in EVs from metabolic syndrome |