Fig. 2

Gene expression analysis of hfcMSCs treated with IFNγ for 7 days. a Effects on the hfcMSC transcriptome by IFNγ stimulation (n = 7 donors in each group), shown as a heatmap depicting the 50 most upregulated genes. b Functional characterization of differentially expressed genes following IFNγ treatment based on Ingenuity Pathway Analysis (IPA). Bar height refers to the number and magnitude of differentially expressed genes in each pathway; black color indicates a positive z-score for the pathway, whereas white indicates a negative z-score, which are calculated based on the expected contribution from up- or downregulated genes. The numbers in brackets refer to (number of differentially expressed genes/number of genes assigned to a particular pathway in IPA). c The bar graph depicts the top ten positive and top ten negative predicted regulators of differential gene expression from the data set. d Upregulation of genes of interest identified from RNA sequencing data, where the respective biological functions of the selected genes are indicated on top of each bar graph. The fold changes depicted in the bar graphs are generated by analysis of difference between the two groups using STAR aligner. A summary of the corrected P values (FDR) for the different genes are presented in table form in Additional file 2