Fig. 1

GLP-1R was decreased after LPS treatment in hCMSCs. RT-qPCR (a). Western blot (b). Values were calculated as the fold change of 2^−△△Ct levels of 0 μg/ml LPS at 24 h (1, 10, and 30 μg/ml at each hour node versus 0 μg/ml, *P < 0.05, **P < 0.01; 0, 1, 10, and 30 μg/ml at 48 h versus at 24 h, ^△P < 0.05; 0, 1, 10, and 30 μg/ml at 72 h versus at 24 h, ^#P < 0.05, ^##P < 0.01). Data were normalized to GAPDH as an internal control. Experiments were conducted in triplicate. A fluorescence microscope (c) detected the impact of 30 μg/m LPS on the distribution of GLP1R-FITC and photographed at × 200. Data are represented as mean ± SD